Research Article
İskemi/Reperfüzyon Hasarı Sonrası İloprost İle Tedavi Edilen Sıçanlarda Tuba Uterina Dokusunun Histopatolojik Değerlendirilmesi
Abstract
Amaç: Sunulan çalışmada sıçanlarda oluşan I/R hasarı sonrasında tuba uterina dokusu üzerinde antioksidan özelliği olan Iloprostun koruyucu etkisinin olup olmadığının değerlendirilmesi amaçlandı.
Gereç ve Yöntemler: Çalışmamızda; 32 dişi Sprag Dawley sıçan kullanıldı. Gruplar sham, torsiyon, detorsiyon ve detorsiyon + ıloprost olarak belirlendi. Sham grubu hariç diğer ratlara torsiyon işlemi yapıldı. Torsiyon grubuna üç saat torsiyon, detorsiyon grubuna 3 saat torsiyon + 3 saat detorsiyon, I/R + ıloprost grubuna 3 saat torsiyon + 3 saat detorsiyon ve detorsiyon başlangıcında 60 dk intravenöz iloprot infüzyonu yapıldı. Histopatolojik değerlendirme için alınan Tuba uterina dokularının histolojik takibi yapıldı. Sonrasında 7 mikron kalınlıkta kesitler alındı ve hematoksilen & Eozin boyası ile boyandı. Alınan kesitler üzerinde ışık mikroskobik inceleme yapıldı.
Bulgular: Tuba uterinada yapılan incelemelerde kontrol grubunda herhangi bir histopatolojik bulguya rastlanmadı. Kontrol grubundan farklı olarak Torsiyon grubunda vasküler tıkanıklık ve epitelde vakuolleşme meydana geldi. Detorsiyon grubunda epitelin bütünlüğünün bozulduğu gözlendi. İloprost uygulanan gruba ait görüntülerde hemarojik alanlar dışında herhangi bir histopatolojik duruma rastlanmadı. Bununla birlikte, bütün gruplarda bağ doku, muskuler alanlar ve seroza tabakaları normaldi.
Sonuç: Antioksidan ve vazodilatör etkisi nedeniyle tedavi amaçlı kullanılan iloprostun sıçan tuba uterina dokusu üzerinde I/R hasarı sonrasında korucu etkisinin olduğu gözlendi. Bu sonuçlar doğrultusunda organın tekrar fonsiyone edilmesi ile torsiyone olan tarafta ektopik gebelik riskinin azaltılabileceği düşünüldü.
Keywords
References
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Drug Design, Development and Therapy. 2016;10:1077–83. 7. McMichael M, Moore RM. Ischemia-reperfusion injury pathophysiology, Part I. Journal of Veterinary Emergency and Critical Care. 2004; 14(4), 231-41. 8. Suleyman B, Albayrak A, Kurt N, Demirci E, Gundogdu C, Aksoy M. The effect of etoricoxib on kidney ischemia-reperfusion injury in rats: a biochemical and immunohistochemical assessment. International Immunopharmacology. 2014;23:179–85. 9. Ibrahim MA, Abdel-Gaber SA, Amin EF, Ibrahim SA, Mohammed RK, Abdelrahman AM. Molecular mechanisms contributing to the protective effect of levosimendan in liver ischemiareperfusion injury. European journal of pharmacology. 2014;741:64–73. 10. Sak ME, Soydinc HE, Sak S, Evsen MS, Alabalik U, Akdemir F, et al. The protective effect of curcumin on ischemia-reperfusion injury in rat ovary. International Journal of Surgery. 2013; 11(9), 967-70. 11. Erer D, Dursun AD, Oktar GL, Iriz E, Zor MH, Elmas C, et al. The effects of iloprost on lung injury induced by skeletal muscle ischemia-reperfusion. Bratislavske lekarske listy. 2014; 115(7): 405-10. 12. Tiryakioglu O, Erkoc K, Tunerir B, Uysal O, Altin HF, Gunes T, et al. The effect of iloprost and N-acetylcysteine on skeletal muscle injury in an acute aortic ischemia-reperfusion model: an experimental study. BioMed research international. 2015; doi: 10.1155/2015/453748. 13. Ergun Y, Koc A, Dolapcioglu K, Akaydin Y, Dogruer G, Kontas T, et al. The protective effect of erythropoietin and dimethylsulfoxide on ischemia-reperfusion injury in rat ovary. European Journal of Obstetrics & Gynecology and Reproductive Biology. 2010; 152(2): 186-90. 14. Yassin MM, Harkin DW, Barros D’Sa AA, Halliday MI, Rowlands BJ. Lower limb ischemia reperfusion injury triggers a systemic inflammatory response and multiple organ dysfunction. World journal of surgery. 2002; 26: 115-21. 15. Cevirme D, Adademir T, Başaran EK, Şavluk ÖF, Elibol A, Şentürk GE, et al. Comparison between iloprost and alprostadil for protection against ischemia/reperfusion injury in a rat model. Turkish journal of medical sciences. 2018; 48(3): 661-9. 16. Erre GL, Passiu G. Antioxidant effect of Iloprost: current knowledge and therapeutic implications for systemic sclerosis. Reumatismo. 2009; 61(2): 90-7. 17. Birukova AA, Wu T, Tian Y, Meliton A, Sarich N, Tian X et al. Iloprost improves endothelial barrier function in lipopolysaccharide-induced lung injury. European Respiratory Journal. 2013; 41: 165-76. 18. Kart C, Aran T, Guven S, Karahan SC, Yulug E. Acute increase in plasma D-dimer level in ovarian torsion: an experimental study. Human Reproduction. 2011; 26(3): 564-8. 19. Ozat M, Gungor T, Barun S, Demirogullari B, Sokmensuer LK, Gulbahar O, et al. The effects of iloprost, a prostacyclin analogue, in experimental ischaemia/ reperfusion injury in rat ovaries. Experimental and Toxicologic Pathology. 2009;61(5):519-27. 20. Kuroda S, Siesjo BK. Reperfusion damage following focal ischemia: pathophysiology and therapeutic windows. Clinical Neuroscience (New York, NY).1997;4(4):199-212. 21. Land WG. The role of postischemic reperfusion injury and other nonantigen dependent inflammatory pathways in transplantation. Transplantation. 2005;79(5):505-14. 22. Baker GL, Corry RJ, Autor AP. Oxygen free radical induced damage in kidneys subjected to warm ischemia and reperfusion: protective effect of superoxide dismutase. Annals of surgery. 1985;202(5):628-41. 23. Sahin FK, Cosar E, Koken G, Toy HK, Buyukbas S. Protective effect of aprotinin on ischemia-reperfusion injury in rat ovary. Journal of Obstetrics and Gynaecology Research. 2008;34(5):794-800. 24. McCord JM. Oxygen-derived free radicals in postischemic tissue injury. New England Journal of Medicine. 1985;312(3):159-63. 25. Schermuly RT, Yilmaz H, Ghofrani HA, Woyda K, Pullamsetti S, Schulz A, et al. Inhaled iloprost reverses vascular remodeling in chronic experimental pulmonary hypertension. American journal of respiratory and critical care medicine. 2005; 172(3): 358-63. 26. Gomberg-Maitland M, Olschewski H. Prostacyclin therapies for the treatment of pulmonary arterial hypertension. European respiratory journal. 2008; 31(4): 891-901. 27. Hsu HH, Rubin LJ. Iloprost inhalation solution for the treatment of pulmonary arterial hypertension. Expert opinion on pharmacotherapy. 2005; 6(11):1921-30.
Year 2020,
Volume: 17 Issue: 1, 280 - 284, 31.03.2020
Abstract
References
- 1. Gündoğdu EC, Karacaoğlu MÜ, Odacılar E, Dayan H, Aksakal GE. Adneksiyel torsiyon olgularında klinik ve cerrahi yaklaşım. Göztepe Tıp Dergisi. 2014; 29(4):214-8. doi:10.5222/J.GOZTEPETRH.2014.214 2. Chang YJ, Yan DC, Kong MS, Wu CT, Chao HC, Luo CC, et al. Adnexal torsion in children. Pediatric emergency care. 2008; 24(8): 534-7. 3. Houry D, Abbott JT. Ovarian torsion: a fifteen-year review. Annals of emergency medicine. 2001;38(2):156-9. 4. Taskin O, Birincioglu M, Aydin A, Buhur A, Burak F, Yilmaz I, et al. The effects of twisted ischaemic adnexa managed by detorsion on ovarian viability and histology: an ischaemia-reperfusion rodent model. Human reproduction. 1998;13(10):2823-7. 5. Kumar V, Cotran RS, Robbins SL. Basic Pathology. 7th ed. Elsevier Publishers, 2003; pages 6-11, 531-3. 6. Yaman TS, Agacayak E, Goruk NY, Icen MS, Turgut A, Alabalık U, et al. Protective effects of honokiol on ischemia/reperfusion injury of rat ovary: an experimental study. Drug Design, Development and Therapy. 2016;10:1077–83. 7. McMichael M, Moore RM. Ischemia-reperfusion injury pathophysiology, Part I. Journal of Veterinary Emergency and Critical Care. 2004; 14(4), 231-41. 8. Suleyman B, Albayrak A, Kurt N, Demirci E, Gundogdu C, Aksoy M. The effect of etoricoxib on kidney ischemia-reperfusion injury in rats: a biochemical and immunohistochemical assessment. International Immunopharmacology. 2014;23:179–85. 9. Ibrahim MA, Abdel-Gaber SA, Amin EF, Ibrahim SA, Mohammed RK, Abdelrahman AM. Molecular mechanisms contributing to the protective effect of levosimendan in liver ischemiareperfusion injury. European journal of pharmacology. 2014;741:64–73. 10. Sak ME, Soydinc HE, Sak S, Evsen MS, Alabalik U, Akdemir F, et al. The protective effect of curcumin on ischemia-reperfusion injury in rat ovary. International Journal of Surgery. 2013; 11(9), 967-70. 11. Erer D, Dursun AD, Oktar GL, Iriz E, Zor MH, Elmas C, et al. The effects of iloprost on lung injury induced by skeletal muscle ischemia-reperfusion. Bratislavske lekarske listy. 2014; 115(7): 405-10. 12. Tiryakioglu O, Erkoc K, Tunerir B, Uysal O, Altin HF, Gunes T, et al. The effect of iloprost and N-acetylcysteine on skeletal muscle injury in an acute aortic ischemia-reperfusion model: an experimental study. BioMed research international. 2015; doi: 10.1155/2015/453748. 13. Ergun Y, Koc A, Dolapcioglu K, Akaydin Y, Dogruer G, Kontas T, et al. The protective effect of erythropoietin and dimethylsulfoxide on ischemia-reperfusion injury in rat ovary. European Journal of Obstetrics & Gynecology and Reproductive Biology. 2010; 152(2): 186-90. 14. Yassin MM, Harkin DW, Barros D’Sa AA, Halliday MI, Rowlands BJ. Lower limb ischemia reperfusion injury triggers a systemic inflammatory response and multiple organ dysfunction. World journal of surgery. 2002; 26: 115-21. 15. Cevirme D, Adademir T, Başaran EK, Şavluk ÖF, Elibol A, Şentürk GE, et al. Comparison between iloprost and alprostadil for protection against ischemia/reperfusion injury in a rat model. Turkish journal of medical sciences. 2018; 48(3): 661-9. 16. Erre GL, Passiu G. Antioxidant effect of Iloprost: current knowledge and therapeutic implications for systemic sclerosis. Reumatismo. 2009; 61(2): 90-7. 17. Birukova AA, Wu T, Tian Y, Meliton A, Sarich N, Tian X et al. Iloprost improves endothelial barrier function in lipopolysaccharide-induced lung injury. European Respiratory Journal. 2013; 41: 165-76. 18. Kart C, Aran T, Guven S, Karahan SC, Yulug E. Acute increase in plasma D-dimer level in ovarian torsion: an experimental study. Human Reproduction. 2011; 26(3): 564-8. 19. Ozat M, Gungor T, Barun S, Demirogullari B, Sokmensuer LK, Gulbahar O, et al. The effects of iloprost, a prostacyclin analogue, in experimental ischaemia/ reperfusion injury in rat ovaries. Experimental and Toxicologic Pathology. 2009;61(5):519-27. 20. Kuroda S, Siesjo BK. Reperfusion damage following focal ischemia: pathophysiology and therapeutic windows. Clinical Neuroscience (New York, NY).1997;4(4):199-212. 21. Land WG. The role of postischemic reperfusion injury and other nonantigen dependent inflammatory pathways in transplantation. Transplantation. 2005;79(5):505-14. 22. Baker GL, Corry RJ, Autor AP. Oxygen free radical induced damage in kidneys subjected to warm ischemia and reperfusion: protective effect of superoxide dismutase. Annals of surgery. 1985;202(5):628-41. 23. Sahin FK, Cosar E, Koken G, Toy HK, Buyukbas S. Protective effect of aprotinin on ischemia-reperfusion injury in rat ovary. Journal of Obstetrics and Gynaecology Research. 2008;34(5):794-800. 24. McCord JM. Oxygen-derived free radicals in postischemic tissue injury. New England Journal of Medicine. 1985;312(3):159-63. 25. Schermuly RT, Yilmaz H, Ghofrani HA, Woyda K, Pullamsetti S, Schulz A, et al. Inhaled iloprost reverses vascular remodeling in chronic experimental pulmonary hypertension. American journal of respiratory and critical care medicine. 2005; 172(3): 358-63. 26. Gomberg-Maitland M, Olschewski H. Prostacyclin therapies for the treatment of pulmonary arterial hypertension. European respiratory journal. 2008; 31(4): 891-901. 27. Hsu HH, Rubin LJ. Iloprost inhalation solution for the treatment of pulmonary arterial hypertension. Expert opinion on pharmacotherapy. 2005; 6(11):1921-30.
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Details
| Primary Language | Turkish |
|---|---|
| Subjects | Obstetrics and Gynaecology |
| Journal Section | Research Article |
| Authors | |
| Publication Date | March 31, 2020 |
| Submission Date | December 28, 2019 |
| Acceptance Date | February 24, 2020 |
| Published in Issue | Year 2020 Volume: 17 Issue: 1 |
