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Investigation of Possibilities of Using Nerium oleander L. Extract as Prebiotic for Lactobacillus acidophilus and Lactobacillus rhamnosus

Yıl 2020, Cilt: 13 Sayı: 3, 1147 - 1157, 31.12.2020
https://doi.org/10.18185/erzifbed.749317

Öz

The use of microorganisms that support human health and stimulate the immune system is gaining more importance. It is thought that probiotic bacterial strains, representing a significant portion of these microorganisms, may be treated in different disease groups. For instance, prevention of gastrointestinal diseases, ulcerative colitis, diarrhea, necrotizing enterocolitis, inflammatory bowel diseases, constipation, urogenital system health, rotavirus diarrhea as well as colon cancer can be prevented using probiotics. The probiotic bacterial strains, have basic needs such as bacterial auto-aggregation and cell surface hydrophobicity. Due to the phytochemicals contained in the Nerium oleander L., this plant has been used as medicinal plants. Such plants, when ingested, contact with the human gastrointestinal microbiome, so beneficial microorganisms can be affected. Thus, present study aimed to investigate the effects of Nerium oleander L. extract on two of the most studied and very well-known probiotic bacteria, Lactobacillus acidophilus and Lactobacillus rhamnosus.
Nerium oleander L. was extracted using ethanol. Probiotics were grown in the presence of this extract. Surface hydrophobicity of probiotics grown in the presence of plant extract was assessed by Microbial Adhesion to Solvents (MATS) Assay, and auto-aggregation was performed.
Auto-aggregation and surface hydrophobicity properties of these bacteria can be modulated by this plant, due to its phytochemicals. Auto-aggregation, as well as surface hydrophobicity were increased or decreased dependent on the dose as the plant extract
The combinations of this plant and a probiotic strain could be next generation functional food, defined as food ingredient that has positive health benefits to the human.

Teşekkür

The authors thank to Chr. Hansen for providing the lactobacilli.

Kaynakça

  • Anandharaj, M., Sivasankari, B., and Rani, P. R., 2014. Effects of Probiotics, Prebiotics, and Synbiotics on Hypercholesterolemia: A Review. Chinese Journal of Biology. 2014: 572754 .
  • Buck, B.L., Altermann, E., Svingerud, T., and Klaenhammer, R.T., 2005. Functional Analysis of Putative Adhesion Factors in Lactobacillus Acidophilus NCFM. Applied and Environmental Microbiology 71 (12): 8344–8351.
  • Celebioglu, H.U., Delsoglio, M., Brix, S., Pessione, E., and Svensson, B. 2018. Plant Polyphenols Stimulate Adhesion to Intestinal Mucosa and Induce Proteome Changes in the Probiotic Lactobacillus Acidophilus NCFM. Molecular Nutrition and Food Research. 62(4): 1700638.
  • Celebioglu, H.U., Ejby, M., Majumder, A., Købler, C., Goh, J.Y., Thorsen, K., Schmidt, B. et al. 2016. Differential Proteome and Cellular Adhesion Analyses of the Probiotic Bacterium Lactobacillus Acidophilus NCFM Grown on Raffinose - an Emerging Prebiotic. Proteomics 16 (9): 1361–1375.
  • Celebioglu, H.U., Olesen, S.V., Prehn, K., Lahtinen, S.J., Brix, S,. Hachem, M.H., and Svensson. B. 2017. Mucin- and Carbohydrate-Stimulated Adhesion and Subproteome Changes of the Probiotic Bacterium Lactobacillus Acidophilus NCFM. Journal of Proteomics 163:102–110.
  • Carmen, C.M,. Meriluoto, J,. and Salminen. S,. 2007. Adhesion and Aggregation Properties of Probiotic and Pathogen Strains. European Food Research and Technology 226 (5): 1065–1073.
  • Del R.B,. Sgorbati, B,. Miglioli, M., and Palenzona, D,. 2000. Adhesion, Autoaggregation and Hydrophobicity of 13 Strains of Bifidobacterium Longum. Letters in Applied Microbiology 31 (6): 438–442.
  • Douglas, L.C,. and Sanders, M.E,. 2008. Probiotics and Prebiotics in Dietetics Practice. Journal of the American Dietetic Association 108 (3): 510–521.
  • Drisko, J.A., Giles, C.K., Bischoff, B.J., 2003. Probiotics in Health Maintenance. Alternative Medicine Review 8 (2): 143–155.
  • Ehrmann, M.A., Kurzak, P., Bauer, J., and Vogel, R.F,. 2002. Characterization of Lactobacilli towards Their Use as Probiotic Adjuncts in Poultry. Journal of Applied Microbiology 92 (5): 966–975.
  • Fotiadis, C. I., Stoidis, C.N., Spyropoulos, B.G, and Zografos, E.D, 2008. Role of Probiotics, Prebiotics and Synbiotics in Chemoprevention for Colorectal Cancer. World Journal of Gastroenterology 14 (42): 6453–6457.
  • García, C.T., Korany, A.M, Bustos, I., Luz P., Cadiñanos, G., Requena, T., Peláez, C., and Cuesta, M.C.M,. 2014. Adhesion Abilities of Dairy Lactobacillus Plantarum Strains Showing an Aggregation Phenotype. Food Research International 57: 44–50.
  • Gayathri, V., Ananthi, S., Chandronitha, C., Ramakrishnan, G., Sundaram, R.L., and Vasanthi, H.R. 2011. Cardioprotective Effect of Nerium Oleander Flower against Isoproterenol-Induced Myocardial Oxidative Stress in Experimental Rats. Journal of Cardiovascular Pharmacology and Therapeutics 16(1):96-104.
  • Gibson, G.R., Scott, K.P., Rastall, R.A., Tuohy, K.M., Hotchkiss, A., Ferrandon, A.D., Gareau, M. 2010. Dietary Prebiotics: Current Status and New Definition. IFIS Functional Foods Bulletin 7 (1): 1–19.
  • Gibson, G.R., Probert, H.M., Loo, J.V., Rastall, R.A., and Roberfroid, M.B. 2004. Dietary Modulation of the Human Colonic Microbiota: Updating the Concept of Prebiotics. Nutrition Research Reviews 17 (2): 259–275.
  • Gill, H., and Prasad, J. 2008. Probiotics, Immunomodulation, and Health Benefits. Advances in Experimental Medicine and Biology, 606: 423–454.
  • González-Rodríguez, I., Ruiz, L., Gueimonde, M., Margolles, A., and Sánchez, B. 2013. Factors Involved in the Colonization and Survival of Bifidobacteria in the Gastrointestinal Tract. FEMS Microbiology Letters 340 (1): 1–10.
  • Greene, J.D., and Klaenhammer, T.R. 1994. Factors Involved in Adherence of Lactobacilli to Human Caco-2 Cells. Applied and Environmental Microbiology 60 (12): 4487-94.
  • Guiné, R.P.F., and Silva, A.C.F., 2016. Probiotics, Prebiotics and Synbiotics. In Functional Foods: Sources, Health Effects and Future Perspectives, Ed. Nelson D.L. , 143–207. USA: Nova Publishers.
  • Hill, C., Guarner, F., Reid, G., Gibson, G.R., Merenstein, D.J., Pot, B., Morelli, L. et al. 2014. Expert Consensus Document: The International Scientific Association for Probiotics and Prebiotics Consensus Statement on the Scope and Appropriate Use of the Term Probiotic. Nature Reviews. Gastroenterology & Hepatology 11: 506–514.
  • Huang, R., Wang, K., and Hu, J. 2016. Effect of Probiotics on Depression: A Systematic Review and Meta-Analysis of Randomized Controlled Trials. Nutrients 8 (8): E483.
  • Ishikawa, H., Matsumoto, S., Ohashi, Y., Imaoka, A., Setoyama, H., Umesaki, Y., Tanaka, R., and Otani, T. 2011. Beneficial Effects of Probiotic Bifidobacterium and Galacto-Oligosaccharide in Patients with Ulcerative Colitis: A Randomized Controlled Study. Digestion 84 (2): 128–133.
  • Kiran, C., and Prasad, D.N., 2014. A Review on: Nerium Oleander Linn. (Kaner). International Journal of Pharmacognosy and Phytochemical Research 6 (3): 593–597.
  • Kleerebezem, M., and Vaughan, E.E. 2009. Probiotic and Gut Lactobacilli and Bifidobacteria: Molecular Approaches to Study Diversity and Activity. Annual Review of Microbiology 63: 269–290.
  • Kos, B., Susković, J., Vuković, S., Simpraga, M., Frece, J., and Matosić, S. 2003. Adhesion and Aggregation Ability of Probiotic Strain Lactobacillus Acidophilus M92. Journal of Applied Microbiology 94 (6): 981–987.
  • Kumar, M., Kumar, A., Nagpal, R., Mohania, D., Behare, D., Verma, V., Kumar, P. et al. 2010. Cancer-Preventing Attributes of Probiotics: An Update. International Journal of Food Sciences and Nutrition 61 (5): 473–496.
  • Laparra, J.M., and Sanz, Y,. 2009. Comparison of in Vitro Models to Study Bacterial Adhesion to the Intestinal Epithelium. Letters in Applied Microbiology 49 (6): 695–701.
  • Lebeer, S., Vanderleyden, J., and De Keersmaecker, S.C.J. 2008. Genes and Molecules of Lactobacilli Supporting Probiotic Action. Microbiology and Molecular Biology Reviews 72 (4): 728–764..
  • Li, Q., Liu, X., Zhou, J., and Wang, Y. 2015. Aggregation and Adhesion Abilities of 18 Lactic Acid Bacteria Strains Isolated from Traditional Fermented Food. International Journal of Agricultural Policy and Research 3 (2): 84–92.
  • Liu, Y., Yang, S.F., Li, Y., Xu, H., Qin, L., and Tay, J.H. 2004. The Influence of Cell and Substratum Surface Hydrophobicities on Microbial Attachment. Journal of Biotechnology 110 (3): 251–256.
  • Nikolic, M., Jovcic, B., Kojic, M., and Topisirovic, L. 2010. Surface Properties of Lactobacillus and Leuconostoc Isolates from Homemade Cheeses Showing Auto-Aggregation Ability. European Food Research and Technology 231 (6): 925–931.
  • Ocaña, V.S., and Nader-Macías, M.E. 2002. Vaginal Lactobacilli: Self- and Co-Aggregating Ability. British Journal of Biomedical Science 59 (4): 183–190.
  • Rashan, L.J., Franke, K., Khine, M.M., Kelter, G., Fiebig, H.H., Neumann, J., and Wessjohann, L.A. 2011. Characterization of the Anticancer Properties of Monoglycosidic Cardenolides Isolated from Nerium Oleander and Streptocaulon Tomentosum. Journal of Ethnopharmacology 134(3): 781-788.
  • Sánchez, B., Urdaci, M.C., and Margolles, A,. 2010. Extracellular Proteins Secreted by Probiotic Bacteria as Mediators of Effects That Promote Mucosa-Bacteria Interactions. Microbiology 156 (11): 3232–3242.
  • Sanders, M.E. 2009. How Do We Know When Something Called ‘ Probiotic ’ Is Really a Probiotic ? A Guideline for Consumers and Health Care Professionals. Functional Food Reviews 1 (1): 3–12.
  • Servin, A.L., and Coconnier, M.H. 2003. Adhesion of Probiotic Strains to the Intestinal Mucosa and Interaction with Pathogens. Best Practice & Research Clinical Gastroenterology 17 (5): 741–754.
  • Siciliano, R.A., and Mazzeo, M.F. 2012. Molecular Mechanisms of Probiotic Action: A Proteomic Perspective. Current Opinion in Microbiology 15 (3): 390–396.
  • Soa, S.S.Y., Wana, M.L.Y., and El-Nezami, H. 2017. Probiotics-Mediated Suppression of Cancer. Current Opinion in Oncology 29 (1): 62–72.
  • van Loosdrecht, M. C., Lyklema, J., Norde, W., Schraa, G., and Zehnder, A. J. 1987. The Role of Bacterial Cell Wall Hydrophobicity in Adhesion. Applied and Environmental Microbiology 53 (8): 1893–1897.
  • Tassell, V., Maxwell L., and Miller, M.J. 2011. Lactobacillus Adhesion to Mucus. Nutrients 3 (5): 613–636.

Lactobacillus acidophilus ve Lactobacillus rhamnosus için Nerium oleander L. Ekstraktının Prebiyotik Olarak Kullanım Olanaklarının Araştırılması

Yıl 2020, Cilt: 13 Sayı: 3, 1147 - 1157, 31.12.2020
https://doi.org/10.18185/erzifbed.749317

Öz

İnsan sağlığını destekleyen ve bağışıklık sistemini uyaran mikroorganizmaların kullanımı gün geçtikçe önem kazanmaktadır. Bu mikroorganizmaların önemli bir bölümünü temsil eden probiyotik bakteri suşlarının farklı hastalık gruplarında tedavi edilebileceği düşünülmektedir. Örneğin, gastrointestinal hastalıkların önlenmesi, ülseratif kolit, ishal, nekrotizan enterokolit, inflamatuar bağırsak hastalıkları, kabızlık, ürogenital sistem sağlığı, rotavirüs ishal ve kolon kanseri probiyotikler kullanılarak önlenebilirliği çalışılmaktadır. Probiyotik bakteri suşları, bakteriyel oto-agregasyon ve hücre yüzeyi hidrofobisitesi gibi temel faktörlere sahiptir. Nerium oleander L.'de bulunan fitokimyasallar nedeniyle, bu bitki tıbbi bitki olarak kullanılagelmiştir. Bu tür bitkiler, insan gastrointestinal mikrobiyomuyla temas eder, bu nedenle yararlı mikroorganizmalar etkilenebilir. Bu nedenle, bu çalışmada Nerium oleander L. ekstresinin en çok çalışılan ve çok iyi bilinen probiyotik bakteriler, Lactobacillus acidophilus ve Lactobacillus rhamnosus üzerindeki etkileri araştırılmıştır.
Nerium oleander L. etanol kullanılarak ekstrakte edilmiştir. Probiyotikler bu ekstraktın varlığında büyütülmüştür. Bitki ekstraktı varlığında büyütülen probiyotiklerin yüzey hidrofobisitesi ve oto-agregasyon testleri yapılmıştır.
Bu bakterilerin oto-agregasyonu ve yüzey hidrofobisite özellikleri, fitokimyasalları nedeniyle bu bitki tarafından modüle edilebilir bulunmuştur. Oto-agregasyon ve yüzey hidrofobisitesi, bitki özütü olarak doza bağlı olarak arttmış veya azalmıştır.
Daha detaylı çalışmalarla birlikte, bu bitkinin ve bir probiyotik türün kombinasyonları, insan için olumlu sağlık yararları olan gıda bileşeni olarak tanımlanan yeni nesil fonksiyonel gıda formülasyonlarını oluşturabilir.

Kaynakça

  • Anandharaj, M., Sivasankari, B., and Rani, P. R., 2014. Effects of Probiotics, Prebiotics, and Synbiotics on Hypercholesterolemia: A Review. Chinese Journal of Biology. 2014: 572754 .
  • Buck, B.L., Altermann, E., Svingerud, T., and Klaenhammer, R.T., 2005. Functional Analysis of Putative Adhesion Factors in Lactobacillus Acidophilus NCFM. Applied and Environmental Microbiology 71 (12): 8344–8351.
  • Celebioglu, H.U., Delsoglio, M., Brix, S., Pessione, E., and Svensson, B. 2018. Plant Polyphenols Stimulate Adhesion to Intestinal Mucosa and Induce Proteome Changes in the Probiotic Lactobacillus Acidophilus NCFM. Molecular Nutrition and Food Research. 62(4): 1700638.
  • Celebioglu, H.U., Ejby, M., Majumder, A., Købler, C., Goh, J.Y., Thorsen, K., Schmidt, B. et al. 2016. Differential Proteome and Cellular Adhesion Analyses of the Probiotic Bacterium Lactobacillus Acidophilus NCFM Grown on Raffinose - an Emerging Prebiotic. Proteomics 16 (9): 1361–1375.
  • Celebioglu, H.U., Olesen, S.V., Prehn, K., Lahtinen, S.J., Brix, S,. Hachem, M.H., and Svensson. B. 2017. Mucin- and Carbohydrate-Stimulated Adhesion and Subproteome Changes of the Probiotic Bacterium Lactobacillus Acidophilus NCFM. Journal of Proteomics 163:102–110.
  • Carmen, C.M,. Meriluoto, J,. and Salminen. S,. 2007. Adhesion and Aggregation Properties of Probiotic and Pathogen Strains. European Food Research and Technology 226 (5): 1065–1073.
  • Del R.B,. Sgorbati, B,. Miglioli, M., and Palenzona, D,. 2000. Adhesion, Autoaggregation and Hydrophobicity of 13 Strains of Bifidobacterium Longum. Letters in Applied Microbiology 31 (6): 438–442.
  • Douglas, L.C,. and Sanders, M.E,. 2008. Probiotics and Prebiotics in Dietetics Practice. Journal of the American Dietetic Association 108 (3): 510–521.
  • Drisko, J.A., Giles, C.K., Bischoff, B.J., 2003. Probiotics in Health Maintenance. Alternative Medicine Review 8 (2): 143–155.
  • Ehrmann, M.A., Kurzak, P., Bauer, J., and Vogel, R.F,. 2002. Characterization of Lactobacilli towards Their Use as Probiotic Adjuncts in Poultry. Journal of Applied Microbiology 92 (5): 966–975.
  • Fotiadis, C. I., Stoidis, C.N., Spyropoulos, B.G, and Zografos, E.D, 2008. Role of Probiotics, Prebiotics and Synbiotics in Chemoprevention for Colorectal Cancer. World Journal of Gastroenterology 14 (42): 6453–6457.
  • García, C.T., Korany, A.M, Bustos, I., Luz P., Cadiñanos, G., Requena, T., Peláez, C., and Cuesta, M.C.M,. 2014. Adhesion Abilities of Dairy Lactobacillus Plantarum Strains Showing an Aggregation Phenotype. Food Research International 57: 44–50.
  • Gayathri, V., Ananthi, S., Chandronitha, C., Ramakrishnan, G., Sundaram, R.L., and Vasanthi, H.R. 2011. Cardioprotective Effect of Nerium Oleander Flower against Isoproterenol-Induced Myocardial Oxidative Stress in Experimental Rats. Journal of Cardiovascular Pharmacology and Therapeutics 16(1):96-104.
  • Gibson, G.R., Scott, K.P., Rastall, R.A., Tuohy, K.M., Hotchkiss, A., Ferrandon, A.D., Gareau, M. 2010. Dietary Prebiotics: Current Status and New Definition. IFIS Functional Foods Bulletin 7 (1): 1–19.
  • Gibson, G.R., Probert, H.M., Loo, J.V., Rastall, R.A., and Roberfroid, M.B. 2004. Dietary Modulation of the Human Colonic Microbiota: Updating the Concept of Prebiotics. Nutrition Research Reviews 17 (2): 259–275.
  • Gill, H., and Prasad, J. 2008. Probiotics, Immunomodulation, and Health Benefits. Advances in Experimental Medicine and Biology, 606: 423–454.
  • González-Rodríguez, I., Ruiz, L., Gueimonde, M., Margolles, A., and Sánchez, B. 2013. Factors Involved in the Colonization and Survival of Bifidobacteria in the Gastrointestinal Tract. FEMS Microbiology Letters 340 (1): 1–10.
  • Greene, J.D., and Klaenhammer, T.R. 1994. Factors Involved in Adherence of Lactobacilli to Human Caco-2 Cells. Applied and Environmental Microbiology 60 (12): 4487-94.
  • Guiné, R.P.F., and Silva, A.C.F., 2016. Probiotics, Prebiotics and Synbiotics. In Functional Foods: Sources, Health Effects and Future Perspectives, Ed. Nelson D.L. , 143–207. USA: Nova Publishers.
  • Hill, C., Guarner, F., Reid, G., Gibson, G.R., Merenstein, D.J., Pot, B., Morelli, L. et al. 2014. Expert Consensus Document: The International Scientific Association for Probiotics and Prebiotics Consensus Statement on the Scope and Appropriate Use of the Term Probiotic. Nature Reviews. Gastroenterology & Hepatology 11: 506–514.
  • Huang, R., Wang, K., and Hu, J. 2016. Effect of Probiotics on Depression: A Systematic Review and Meta-Analysis of Randomized Controlled Trials. Nutrients 8 (8): E483.
  • Ishikawa, H., Matsumoto, S., Ohashi, Y., Imaoka, A., Setoyama, H., Umesaki, Y., Tanaka, R., and Otani, T. 2011. Beneficial Effects of Probiotic Bifidobacterium and Galacto-Oligosaccharide in Patients with Ulcerative Colitis: A Randomized Controlled Study. Digestion 84 (2): 128–133.
  • Kiran, C., and Prasad, D.N., 2014. A Review on: Nerium Oleander Linn. (Kaner). International Journal of Pharmacognosy and Phytochemical Research 6 (3): 593–597.
  • Kleerebezem, M., and Vaughan, E.E. 2009. Probiotic and Gut Lactobacilli and Bifidobacteria: Molecular Approaches to Study Diversity and Activity. Annual Review of Microbiology 63: 269–290.
  • Kos, B., Susković, J., Vuković, S., Simpraga, M., Frece, J., and Matosić, S. 2003. Adhesion and Aggregation Ability of Probiotic Strain Lactobacillus Acidophilus M92. Journal of Applied Microbiology 94 (6): 981–987.
  • Kumar, M., Kumar, A., Nagpal, R., Mohania, D., Behare, D., Verma, V., Kumar, P. et al. 2010. Cancer-Preventing Attributes of Probiotics: An Update. International Journal of Food Sciences and Nutrition 61 (5): 473–496.
  • Laparra, J.M., and Sanz, Y,. 2009. Comparison of in Vitro Models to Study Bacterial Adhesion to the Intestinal Epithelium. Letters in Applied Microbiology 49 (6): 695–701.
  • Lebeer, S., Vanderleyden, J., and De Keersmaecker, S.C.J. 2008. Genes and Molecules of Lactobacilli Supporting Probiotic Action. Microbiology and Molecular Biology Reviews 72 (4): 728–764..
  • Li, Q., Liu, X., Zhou, J., and Wang, Y. 2015. Aggregation and Adhesion Abilities of 18 Lactic Acid Bacteria Strains Isolated from Traditional Fermented Food. International Journal of Agricultural Policy and Research 3 (2): 84–92.
  • Liu, Y., Yang, S.F., Li, Y., Xu, H., Qin, L., and Tay, J.H. 2004. The Influence of Cell and Substratum Surface Hydrophobicities on Microbial Attachment. Journal of Biotechnology 110 (3): 251–256.
  • Nikolic, M., Jovcic, B., Kojic, M., and Topisirovic, L. 2010. Surface Properties of Lactobacillus and Leuconostoc Isolates from Homemade Cheeses Showing Auto-Aggregation Ability. European Food Research and Technology 231 (6): 925–931.
  • Ocaña, V.S., and Nader-Macías, M.E. 2002. Vaginal Lactobacilli: Self- and Co-Aggregating Ability. British Journal of Biomedical Science 59 (4): 183–190.
  • Rashan, L.J., Franke, K., Khine, M.M., Kelter, G., Fiebig, H.H., Neumann, J., and Wessjohann, L.A. 2011. Characterization of the Anticancer Properties of Monoglycosidic Cardenolides Isolated from Nerium Oleander and Streptocaulon Tomentosum. Journal of Ethnopharmacology 134(3): 781-788.
  • Sánchez, B., Urdaci, M.C., and Margolles, A,. 2010. Extracellular Proteins Secreted by Probiotic Bacteria as Mediators of Effects That Promote Mucosa-Bacteria Interactions. Microbiology 156 (11): 3232–3242.
  • Sanders, M.E. 2009. How Do We Know When Something Called ‘ Probiotic ’ Is Really a Probiotic ? A Guideline for Consumers and Health Care Professionals. Functional Food Reviews 1 (1): 3–12.
  • Servin, A.L., and Coconnier, M.H. 2003. Adhesion of Probiotic Strains to the Intestinal Mucosa and Interaction with Pathogens. Best Practice & Research Clinical Gastroenterology 17 (5): 741–754.
  • Siciliano, R.A., and Mazzeo, M.F. 2012. Molecular Mechanisms of Probiotic Action: A Proteomic Perspective. Current Opinion in Microbiology 15 (3): 390–396.
  • Soa, S.S.Y., Wana, M.L.Y., and El-Nezami, H. 2017. Probiotics-Mediated Suppression of Cancer. Current Opinion in Oncology 29 (1): 62–72.
  • van Loosdrecht, M. C., Lyklema, J., Norde, W., Schraa, G., and Zehnder, A. J. 1987. The Role of Bacterial Cell Wall Hydrophobicity in Adhesion. Applied and Environmental Microbiology 53 (8): 1893–1897.
  • Tassell, V., Maxwell L., and Miller, M.J. 2011. Lactobacillus Adhesion to Mucus. Nutrients 3 (5): 613–636.
Toplam 40 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Konular Mühendislik
Bölüm Makaleler
Yazarlar

Hasan Ufuk Celebioglu 0000-0001-7207-2730

Aylin Kesici Bu kişi benim 0000-0001-8975-923X

Recep Taş 0000-0002-3743-7770

Yayımlanma Tarihi 31 Aralık 2020
Yayımlandığı Sayı Yıl 2020 Cilt: 13 Sayı: 3

Kaynak Göster

APA Celebioglu, H. U., Kesici, A., & Taş, R. (2020). Investigation of Possibilities of Using Nerium oleander L. Extract as Prebiotic for Lactobacillus acidophilus and Lactobacillus rhamnosus. Erzincan University Journal of Science and Technology, 13(3), 1147-1157. https://doi.org/10.18185/erzifbed.749317