Research Article
Year 2021,
Volume: 16 Issue: 2, 219 - 225, 07.07.2021
Abstract
Bakteriyel vajinosis (BV) puberte dönemindeki kadınlarda en sık görülen alt genital sistem yakınmasıdır. Vajendeki ekosistemin bozulması sonucu koruyucu laktobasillerin azalması ve anaerob bakteri sayısının aşırı derecede artması sonucu ortaya çıkar.
Bu çalışmada asemptomatik 40 kadından alınan vajinal (VSÖ) ve rektal sürüntü örnekleri (RSÖ) laktobasil insidansı ve türlerinin dağılımını tespit için sellektif kültür yöntemleri kullanılarak değerlendirilmiştir. Ayrıca laktobasillerin karekterizasyonunda fenotipik yöntemlerin yanı sıra sekans bazlı RAPD-PCR yöntemi denenmiştir.
Çalışmaya dahil edilen kadınların Ison & Hay kriterlerine göre değerlendirilmesi sonucu 5 (%15)’inin asemptomatik BV bulgularına sahip oldukları görülmüştür. Kadınların vajen ve rektum sürüntü örneklerinden 132 farklı bakteri kolonisi laktobasil şüpheli bulunmuş, kristal yöntemi ile bunlardan 111’inin 11 farklı türe ait laktobasil olduğu, RAPD-PCR ile yapılan tiplendirmede ise sadece 101’inin 9 farklı tür laktobasil olduğu tespit edilmiştir. RAPD-PCR sonucları baz alındığında 28 (%70) kadında vajen, 31 (%77.5) kadında da rektal sürüntü örneklerinde en az bir laktobasil türünün kolonize olduğu, vajen de en sık kolonize olan türün 10 izolat (%21) ile L. gasseri olduğu, bu türün rektumdan 2. sıklıkta izole edilen tür olup kadınların 6’sında (%29.8) hem vajen hem de rektum örneklerinde kolonize olduğu görülmüştür.
Sonuç olarak, vajen ve rektumunda kolonize olan laktobasil türleri arasında yüksek oranda benzerlik olduğu görülmüş, olup vajene kolonize olan laktobasillerin rektum kökenli oldukları çalışmayı desteklenmiştir.
Keywords
References
- 1. Roberto RR, Hassan SS, Gajer P, Tarca A.L, Fadrosh D.W, Nikita L. et al The composition and stability of the vaginal microbiota of normal pregnant women is different from that of non-pregnant women. Microbiome 2014; 2:4.
- 2. Amabebe E. and Anumba D.O.C. The Vaginal Microenvironment: The Physiologic Role of Lactobacilli. Front. Med 2018; 5:181.
- 3. Gaspar C, Donders G.G, Oliveira R.P, Queiroz J.A, Tomaz C, Oliveira J.M et al. Bacteriocin production of the probiotic Lactobacillus acidophilus KS400. AMB Expr 2018; 8:153.
- 4. Mitchell C, Srinivasan S, Zhan X, Wu M, Reed S, Guthrie K et al. Associations between serum estrogen, vaginal microbiota and vaginal glycogen in postmenopausal women. Am J Obstet Gynecol 2016; 215: S827.
- 5. Smith SB & Ravel J. The vaginal microbiota, host defence and reproductive physiology. J Physiol 2016; 595: 451.
- 6. Fosch SE, Ficoseco CA, Mac’esin A, Cocucci S, Macias MEFN and Perazzi BE. Contraception: Influence on Vaginal Microbiota and Identification of Vaginal Lactobacilli Using MALDI-TOF MS and 16S rDNA Sequencing. The Open Microbiology Journal 2018; 12, 218-229.
- 7. O’Hanlon DE, Come RA and Moench TR. Vaginal pH measured in vivo: lactobacilli determine pH and lactic acid concentration. BMC Microbiology 2019; 19:13.
- 8. Tachedjian G, O'Hanlon DE & Ravel J. The implausible "in vivo" role of hydrogen peroxide as an antimicrobial factor produced by vaginal microbiota. Microbiome 2018; 6: 29.
- 9. Yen S. Shafer MA. Monacada J. Campbell CJ. Flinn SD. Boyer CB. Bacterial vaginosis in sexually experinced and non-sexually experinced young women enteringthe militaryy. Obsest Gynecol 2003; 102:927-933.
- 10. Murphy K & Mitchell CM. The interplay of host immunity, environment and the risk of bacterial vaginosis and associated reproductive health outcomes. J Infect Dis 2016; 214:29.
- 11. Bautista CT, Wurapa E, Sateren WB, Morris S, Hollingsworth B, Sanchez JL. Bacterial vaginosis: a synthesis of the literature on etiology, prevalence, risk factors, and relationship with chlamydia and gonorrhea infections. Mil Med Res 2016; 3: 4.
- 12. Dols J A.M, Smit Pieter W, Kort Remco, Reid Gregor, Schuren F.H.J, Tempelman H et al. Microarraybased identification of clinically relevant vajinal bacteria in relation to bacterial vajinozis. American Journal of Obstetrics and Gynecology 2011; 204(4): 305.
- 13. Onderdonk AB, Delaney ML & Fichorova RN The human microbiome during bacterial vaginosis. Clin Microbiol Rev 2016; 29: 223-238. 14. Ison CA, Hay PE. Validation of a simplified grading of Gram stained vaginal smears for use in genitourinary medicine clinics. Sex Transm Infect. 2002; 78(6):413–415.
- 15. VanDaele S, Vaneechoutte M, Boeck K, Knoop C, Malfroot A, Lebecque P et al. Survey of Pseudomonas aeruginosa genotypes in colonised cystic fibrosis patients. Eur Respir J 2006; 28(4):740-747.
- 16. Baele M, Baele P, Vaneechoutte M, Storms V, Butaye P, Devriese LA et al. Application of tRNA intergenic spacer PCR for identification of Enterococcus species. J Clin Microbiol 2000; 38(11):4201-4207.
- 17. Veer C, Houdt RV, Dam AV, Vries H, Sylvia Bruisten. Accuracy of a commercial multiplex PCR for the diagnosis of bacterial vaginosis. Journal of Medical Microbiology 2018; 67:1265–1270.
- 18. Famularo G, Pieluigi M, Coccia R, Mastroiacovo P, Simone C. Microecelogy, bacterial vaginozis and probiotics: perspectives for bacteriotherapy. Medical Hypotheses 2001; 56(4): 421430.
- 19. Antonio MAD, Rabe LK, Hillier SL. Colonization of the Rectum by Lactobacillus Species and Decreased Risk of Bacterial Vaginosis. JID 2005;192.
- 20. Gustafsson RJ, Ahrné S, Jeppsson B, Benoni C, Olsson C, Stjernquist M et al. The Lactobacillus flora in vagina and rectum of fertile and postmenopausal healthy Swedish women. BMC Women’s Health 2011; 11:17.
- 21. Petricevic L, Domig KJ, Nierscher FJ, Sandhofer MJ, Krondorfer I, Kneifel W, H. Kiss. Differences in the vaginal lactobacilli of postmenopausal women and influence of rectal lactobacilli. Clımacterıc 2013; 16:356–361.
- 22. Kılıç AO, Pavlova SI, Alpay Ş, Kılıç SS, Tao L. Comparative study of vajinal Lactobacillus phages isolated from women in the United States and Turkey: Prevalance, morphology, host range and DNA homology. Clin. Dian. Lab. Immun 2001; 8:31-39.
- 23. Aslım B, Kılıç E. Some probiotic properties of vajinal lactobacilli isolated from healty women. Jpn. Infect. Dis 2006; 59: 249-253.
Year 2021,
Volume: 16 Issue: 2, 219 - 225, 07.07.2021
Abstract
References
- 1. Roberto RR, Hassan SS, Gajer P, Tarca A.L, Fadrosh D.W, Nikita L. et al The composition and stability of the vaginal microbiota of normal pregnant women is different from that of non-pregnant women. Microbiome 2014; 2:4.
- 2. Amabebe E. and Anumba D.O.C. The Vaginal Microenvironment: The Physiologic Role of Lactobacilli. Front. Med 2018; 5:181.
- 3. Gaspar C, Donders G.G, Oliveira R.P, Queiroz J.A, Tomaz C, Oliveira J.M et al. Bacteriocin production of the probiotic Lactobacillus acidophilus KS400. AMB Expr 2018; 8:153.
- 4. Mitchell C, Srinivasan S, Zhan X, Wu M, Reed S, Guthrie K et al. Associations between serum estrogen, vaginal microbiota and vaginal glycogen in postmenopausal women. Am J Obstet Gynecol 2016; 215: S827.
- 5. Smith SB & Ravel J. The vaginal microbiota, host defence and reproductive physiology. J Physiol 2016; 595: 451.
- 6. Fosch SE, Ficoseco CA, Mac’esin A, Cocucci S, Macias MEFN and Perazzi BE. Contraception: Influence on Vaginal Microbiota and Identification of Vaginal Lactobacilli Using MALDI-TOF MS and 16S rDNA Sequencing. The Open Microbiology Journal 2018; 12, 218-229.
- 7. O’Hanlon DE, Come RA and Moench TR. Vaginal pH measured in vivo: lactobacilli determine pH and lactic acid concentration. BMC Microbiology 2019; 19:13.
- 8. Tachedjian G, O'Hanlon DE & Ravel J. The implausible "in vivo" role of hydrogen peroxide as an antimicrobial factor produced by vaginal microbiota. Microbiome 2018; 6: 29.
- 9. Yen S. Shafer MA. Monacada J. Campbell CJ. Flinn SD. Boyer CB. Bacterial vaginosis in sexually experinced and non-sexually experinced young women enteringthe militaryy. Obsest Gynecol 2003; 102:927-933.
- 10. Murphy K & Mitchell CM. The interplay of host immunity, environment and the risk of bacterial vaginosis and associated reproductive health outcomes. J Infect Dis 2016; 214:29.
- 11. Bautista CT, Wurapa E, Sateren WB, Morris S, Hollingsworth B, Sanchez JL. Bacterial vaginosis: a synthesis of the literature on etiology, prevalence, risk factors, and relationship with chlamydia and gonorrhea infections. Mil Med Res 2016; 3: 4.
- 12. Dols J A.M, Smit Pieter W, Kort Remco, Reid Gregor, Schuren F.H.J, Tempelman H et al. Microarraybased identification of clinically relevant vajinal bacteria in relation to bacterial vajinozis. American Journal of Obstetrics and Gynecology 2011; 204(4): 305.
- 13. Onderdonk AB, Delaney ML & Fichorova RN The human microbiome during bacterial vaginosis. Clin Microbiol Rev 2016; 29: 223-238. 14. Ison CA, Hay PE. Validation of a simplified grading of Gram stained vaginal smears for use in genitourinary medicine clinics. Sex Transm Infect. 2002; 78(6):413–415.
- 15. VanDaele S, Vaneechoutte M, Boeck K, Knoop C, Malfroot A, Lebecque P et al. Survey of Pseudomonas aeruginosa genotypes in colonised cystic fibrosis patients. Eur Respir J 2006; 28(4):740-747.
- 16. Baele M, Baele P, Vaneechoutte M, Storms V, Butaye P, Devriese LA et al. Application of tRNA intergenic spacer PCR for identification of Enterococcus species. J Clin Microbiol 2000; 38(11):4201-4207.
- 17. Veer C, Houdt RV, Dam AV, Vries H, Sylvia Bruisten. Accuracy of a commercial multiplex PCR for the diagnosis of bacterial vaginosis. Journal of Medical Microbiology 2018; 67:1265–1270.
- 18. Famularo G, Pieluigi M, Coccia R, Mastroiacovo P, Simone C. Microecelogy, bacterial vaginozis and probiotics: perspectives for bacteriotherapy. Medical Hypotheses 2001; 56(4): 421430.
- 19. Antonio MAD, Rabe LK, Hillier SL. Colonization of the Rectum by Lactobacillus Species and Decreased Risk of Bacterial Vaginosis. JID 2005;192.
- 20. Gustafsson RJ, Ahrné S, Jeppsson B, Benoni C, Olsson C, Stjernquist M et al. The Lactobacillus flora in vagina and rectum of fertile and postmenopausal healthy Swedish women. BMC Women’s Health 2011; 11:17.
- 21. Petricevic L, Domig KJ, Nierscher FJ, Sandhofer MJ, Krondorfer I, Kneifel W, H. Kiss. Differences in the vaginal lactobacilli of postmenopausal women and influence of rectal lactobacilli. Clımacterıc 2013; 16:356–361.
- 22. Kılıç AO, Pavlova SI, Alpay Ş, Kılıç SS, Tao L. Comparative study of vajinal Lactobacillus phages isolated from women in the United States and Turkey: Prevalance, morphology, host range and DNA homology. Clin. Dian. Lab. Immun 2001; 8:31-39.
- 23. Aslım B, Kılıç E. Some probiotic properties of vajinal lactobacilli isolated from healty women. Jpn. Infect. Dis 2006; 59: 249-253.
There are 22 citations in total.
Details
| Primary Language | Turkish |
|---|---|
| Subjects | Health Care Administration |
| Journal Section | Araştırma Makaleleri |
| Authors | |
| Publication Date | July 7, 2021 |
| Submission Date | August 18, 2020 |
| Acceptance Date | November 7, 2020 |
| Published in Issue | Year 2021 Volume: 16 Issue: 2 |
